The manuscript describes the design of a simple LED illuminator for fluorescence microscopy of biological objects. As the light source, high-brightness semiconductor diodes were chosen that provide stable low-noise light. This distinguishes them from traditional light sources, such as mercury lamps. The presented light source can be used to record low-amplitude fluorescent signals in studies of excitable cells performed using calcium or potential-sensitive dyes. The illuminator is made of inexpensive, easily accessible components.
Keywords: high-brightness LEDs, illuminator, fluorescence, microscopy